The Microflora Structure in the Digestive Tract, Culture Water, and Feed of Hybrid Grouper (Epinephelus fuscoguttatus♀ × E. polyphekadion♂) Cultured in an Outdoor Pond Based on a High-Throughput Sequencing Technique

Nutrition, disease, and general wellbeing can be affected by the microbial communities associated with digestive tracts of aquaculture species. Different sections species’ have distinctive surfaces structures, which change communities. The present study examined composition distribution bacterial species in intestine hybrid grouper (Epinephelus fuscoguttatus E. polyphekadion) its environment. Using high-throughput pyrosequencing, a 16S rRNA sequence analysis was performed on foregut, midgut, hindgut, as well cultured water feed. There were 610,452 sequences obtained from five components (foregut, water, feed). Among operational taxa (OTUs), 506 them detected 605 510 573 feed samples. A total 113 5 annotation revealed that intestinal dominated Proteobacteria (67.3%–73.7%), Firmicutes (8.4%–14.0%), Actinobacteria (6.9%–10.5%). In culture predominant (36.3%), (30.0%), Planctomycetes (14.0%). Acinetobacter (1.4%–17.9%) Photobacterium (32.0%–57.5%) intestine. (3.6%) Mycobacterium (7.1%) bacteria. contained potentially pathogenic bacteria: Pseudomonas, Flavobacterium, Escherichia coli, Aeromonas bacteria, Vibrio. highest proportion Vibrio found (1.7%), while Pseudomonas midgut (2.6%). Six potential probiotics (Lactococcus, Streptococcus, Bdellovibrio, Lactobacillus, Bacillus, Bacteroides). Aquaculture intestines Bacteroides, Lactobacillus. According to findings, flora is closely correlated pond Results provide an experimental basis for controlled breeding groupers regulation their microecological processes environment deepen our understanding population healthy groupers.